Abcam, ab16048, Anti-Lamin B1 antibody - Nuclear Envelope Marker

Size: 100µg
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) is a rabbit polyclonal antibody detecting Lamin B1 in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Over 1180 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Product details:
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P and WB.
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) was first used in a scientific publication in 1997 and has been cited over 1182 times in peer reviewed journals. It's performance in Western blot and immunofluorescence in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) has high sensitivity and specificity.
The specificity of Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) has been confirmed by ICC/IF testing in Lamin B1 knockout HAP1 cells.
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) has 108 independent reviews from customers.
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) specifically detects Lamin B1 (UniProt ID: P14733; Molecular weight: 67kDa) and is sold in 100 ug selling sizes.
Top cited with >1500 citations. Lamin B1 is essential for studying nuclear envelope structure and function, as well as investigating diseases like laminopathies and certain cancers

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lamin B1 also known as LMNB1 is a protein component of the nuclear lamina a dense fibrillar network inside the nuclear envelope. It plays a critical structural role in maintaining nuclear integrity. Lamin B1 typically has a molecular weight of approximately 67 kDa. This protein is expressed in the cells of almost all tissues contributing to the maintenance and organization of the nuclear envelope. Researchers often use Western blot techniques to detect and analyze Lamin B1 size and expression levels in various biological samples.
Biological function summary
The Lamin B1 protein supports nuclear stability and regulates chromatin organization. It is a component of the nuclear lamina complex interacting with other lamin proteins such as lamin A and lamin C to form a structured network. This network is essential for chromatin tethering and gene expression regulation influencing cellular processes at the transcriptional level. Its interactions with chromatin and other nuclear components highlight its significance in the overall functional architecture of the cell nucleus.
Pathways
Lamin B1 interacts with molecules involved in the mitotic spindle assembly and DNA replication pathways. It plays an essential role in mitosis where it helps in the disassembly and reassembly of the nuclear envelope. Lamin B1's interactions with proteins like lamin A/C and emerin highlight its participation in nuclear membrane dynamics and chromatin organization during the cell cycle.
Abnormalities in Lamin B1 levels or function associate with conditions like autosomal dominant leukodystrophy and certain types of cancer. Misregulation or mutation of LMNB1 can alter nuclear stability and gene expression contributing to disease pathology. Lamin B1 also connects with proteins like emerin where the disrupted interaction may lead to nuclear envelope-related muscular dystrophies. Understanding these connections is vital for elucidating the molecular basis of these diseases.