Abcam, ab161103, Recombinant Human Rag A protein

Size: 10µg
Recombinant Human Rag A protein is a Human Full Length protein, in the 1 to 313 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q7L523,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Product details:
This product was previously labelled as RRAGA.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rag A is a small GTPase also known as Ras-related GTP-binding protein A. It plays a role in cellular growth and proliferation. The protein operates by binding to GTP and GDP functioning as a molecular switch. Rag A's molecular weight is approximately 42 kDa. It is expressed in many tissues throughout the body indicating its widespread role in cellular processes.
Biological function summary
Rag A interacts with amino acids and nutrient signaling by activating the mTORC1 complex a central regulator of cell growth. It ensures correct localization of mTORC1 to the lysosomal surface facilitating its activation. The mTORC1 complex includes other proteins such as RAPTOR and mLST8 which collectively modulate cell metabolism and protein synthesis. Rag A's participation is essential for sensing nutrients and the energy state within cells.
Pathways
With regards to pathways Rag A is involved in the mTOR signaling pathway and the autophagy-lysosome pathway. These two pathways are important to maintaining cellular homeostasis and responding to nutrient availability. Rag A directly interacts with Rag C another protein in this pathway to relay the presence of amino acids to mTORC1. Through these interactions Rag A aids in the regulation of cellular growth energy management and metabolism in response to environmental cues.
Rag A has connections to conditions like cancer and metabolic disorders. Abnormal activation of the mTOR pathway often involving Rag A can lead to uncontrolled cell proliferation seen in cancers. Additionally the protein's role in nutrient sensing links Rag A to metabolic disorders like diabetes. Alterations in Rag A activity can influence the mTORC1 pathway and misregulate associated proteins such as TSC2 contributing to disease development and progression.