Abcam, ab161644, Recombinant Human Cela3b protein

Size: 10µg
Recombinant Human Cela3b protein is a Human Full Length protein, in the 19 to 270 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P08861,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The CELA3B protein also known as elastase 3B has a significant role in the digestive system. It is a serine protease with a molecular mass of approximately 28 kDa. CELA3B is expressed mainly in the pancreas and is secreted into the small intestine. Its primary mechanical function involves breaking down proteins into peptides facilitating digestion in the intestinal lumen.
Biological function summary
The process of protein digestion relies heavily on the proteolytic activity of CELA3B. The enzyme functions as a monomer and does not form a complex. Its action contributes to the efficient processing of dietary proteins making them available for further breakdown by other proteases like trypsin and chymotrypsin. CELA3B's role in protein digestion is essential for nutrient absorption and overall digestive health.
Pathways
Protein digestion pathways prominently feature the action of CELA3B. It closely interacts with trypsin and chymotrypsin activating these enzymes through initial protein cleavage. This enzyme cascade results in a coordinated protein breakdown. Moreover CELA3B is an integral part of the digestive enzyme pathway which ensures the sequential activation and function of proteolytic enzymes necessary for effective metabolism of dietary proteins.
Impaired CELA3B activity or expression links with pancreatic diseases such as chronic pancreatitis and exocrine pancreatic insufficiency. These conditions often exhibit reduced levels of CELA3B leading to digestive problems and nutrient malabsorption. The enzyme's dysregulation can also relate to cystic fibrosis where the thickening of digestive secretions affects the activity of pancreatic enzymes including CELA3B.