Abcam, ab162135, Recombinant Human SMPDL3B protein

Size: 10µg
Recombinant Human SMPDL3B protein is a Human Full Length protein, in the 1 to 373 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q92485,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMPDL3B also known as sphingomyelin phosphodiesterase-like 3B is an enzyme with a mass of approximately 50 kDa. This protein belongs to the acid sphingomyelinase family and is primarily involved in the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. SMPDL3B is expressed in several tissues including the liver kidney and immune cells where it plays an important role in maintaining cellular sphingolipid metabolism.
Biological function summary
SMPDL3B regulates various cellular processes such as apoptosis proliferation and differentiation by controlling ceramide levels. It does not typically function as part of a larger protein complex but interacts with membrane components to exert its enzymatic activity. SMPDL3B modulates signal transduction pathways that are sensitive to changes in membrane lipid composition influencing cellular responses to stress and immune signals.
Pathways
SMPDL3B is involved in the sphingolipid metabolism pathway and the cholesterol metabolism pathway. It interacts with other sphingolipid enzymes like acid ceramidase which further regulates ceramide levels. SMPDL3B affects the activation of signaling proteins like protein kinase C which links it to various cellular responses. Changes in sphingolipid metabolism can influence pathways linked to cell survival and inflammation.
SMPDL3B plays a role in conditions such as cancer and atherosclerosis. Altered SMPDL3B expression or activity can lead to imbalances in ceramide levels contributing to the pathogenesis of these diseases. In cancer SMPDL3B influences tumor progression through its impact on cell growth and apoptosis interacting with proteins such as Bcl-2. In atherosclerosis it affects lipid accumulation and inflammation relating to proteins like low-density lipoprotein (LDL).