Abcam, ab164670, Recombinant Human PHF5A protein

Size: 10µg
Recombinant Human PHF5A protein is a Human Full Length protein, in the 1 to 110 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q7RTV0,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHF5A also called PHD finger protein 5A is a part of the spliceosome complex. This protein weighs around 13 kDa and is involved in regulating RNA splicing. PHF5A is mainly expressed in the cell nucleus where it plays a role in the splicing machinery. The protein interacts with components of the spliceosome facilitating assembly and function which are necessary for proper RNA splicing events.
Biological function summary
PHF5A functions as an important component of the splicing factor complex. It supports the recognition and selection of splice sites during pre-mRNA processing. PHF5A forms part of the U2 small nuclear ribonucleoprotein complex which is involved in the splicing of pre-messenger RNA. This complex interaction suggests its role in the accurate modification of pre-mRNA into a mature mRNA an important process in gene expression regulation.
Pathways
PHF5A takes part in the RNA splicing pathway. This pathway contains critical interactions that contribute to the mRNA processing necessary for gene expression. PHF5A is related to other spliceosome components such as SF3b which work together in the splicing process. Through these interactions PHF5A supports gene expression regulation which is important for cellular function and homeostasis.
PHF5A is associated with cancer particularly glioblastoma. The protein's role in RNA splicing links it to the misregulation observed in these cancers. PHF5A interacts with splicing factors whose altered expression or function can contribute to tumorigenesis. Disruptions in its pathway might affect the expression of genes involved in cell cycle regulation playing a significant role in disease progression.