Abcam, ab16584, Anti-Orexin Prepro antibody

Size: 50µL
Rabbit Polyclonal Orexin antibody. Suitable for ELISA, WB and reacts with Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified by antigen affinity chromatography., Storage buffer-pH: 7.4Constituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The orexin prepro also known as prepro-orexin is a precursor molecule with a mass of approximately 16-18 kDa. It undergoes proteolytic cleavage to form orexin-A and orexin-B neuropeptides. These peptides play an essential role in maintaining wakefulness and energy homeostasis. The orexin prepro is mainly expressed in the hypothalamus particularly in the lateral hypothalamic area where it acts on orexin receptors distributed widely across the brain.
Biological function summary
The orexin neuropeptides derived from the orexin prepro are important for regulating sleep-wake cycles appetite and arousal. While not part of a larger complex orexins are significant modulators of other neurotransmitter systems including noradrenaline dopamine and serotonin. These interactions influence many physiological functions further demonstrating the importance of orexins in maintaining overall body balance.
Pathways
Orexin prepro-derived neuropeptides engage in the sleep-wake regulation and energy homeostasis pathways. They interact directly with orexin receptors such as OX1R and OX2R to affect these pathways. The insulin signaling pathway also involves orexin peptides linking them with metabolic regulation. Other proteins such as leptin and ghrelin interact with orexins indicating the overlap of neuronal and metabolic pathways in which orexins play.
Disruptions in orexin signaling have strong associations with narcolepsy and obesity. Narcolepsy characterized by fragmented sleep-wake cycles shows a loss of orexin-producing neurons. Obesity relates to orexin through its involvement in appetite regulation and energy balance with dysregulation leading to weight gain. Orexin peptides and their receptors are potential therapeutic targets for these conditions creating connections with other proteins like melanin-concentrating hormone involved in energy homeostasis and sleep regulation.