Abcam, ab16667, Anti-Ki67 antibody [SP6]

Size: 20µL / 100µL / 500µL / 1mL
Anti-Ki67 antibody [SP6] (ab16667) is a rabbit monoclonal antibody detecting Ki67 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, mIHC . Suitable for Human, Mouse, Rat . - Clone SP6 is the most cited clone to Ki67 - KO validated for confirmed specificity - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP6,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), ICC/IF, IHC-P, mIHCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:C-terminus,
Specificity:Ki67 is mainly expressed in proliferating cells. For normal tissue samples (e.g., liver, kidney), no staining may be typically observed due to low level of proliferation and little expression of Ki67. For malignant tissue samples (e.g., colon carcinoma, breast carcinoma), it is more easily to find Ki67 in the proliferating cells of these tissues (PMID: 6206131, 10653597, 34183782).FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the Product Protocol section.

Product details:
Anti-Ki67 antibody [SP6] (ab16667) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB and mIHC.
Anti-Ki67 antibody [SP6] (ab16667) was first used in a scientific publication in 1977 and has been cited over 2748 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Ki67 antibody [SP6] (ab16667) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Ki67 antibody [SP6] (ab16667) has been confirmed by Western Blot testing in Ki67 knockout HeLa cells (
ab263762
Anti-Ki67 antibody [SP6] (ab16667) has 123 independent reviews from customers.
Anti-Ki67 antibody [SP6] (ab16667) specifically detects Ki67 (UniProt ID: P46013; Molecular weight: 359kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone SP6 -
ab197547
Antibody clone SP6 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, PE, APC (
ab281847
ab281928
ab282173
ab314285
Anti-Ki67 antibody [SP6] (ab16667) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Ki-67 is a protein that serves as a marker for cell proliferation in oncology. It is widely used to assess the growth rate of cancer cells, helping to determine the aggressiveness of tumors and guide treatment decisions. High levels of Ki-67 are often associated with more aggressive cancers and poorer prognosis
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.
Biological function summary
The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.
Pathways
Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.
Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.