Abcam, ab167154, Anti-TRIM25/EFP antibody [EPR7315]

Size: 100µL / 1mL
Anti-TRIM25/EFP antibody [EPR7315] (ab167154) is a rabbit monoclonal antibody detecting TRIM25/EFP in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7315,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, Flow Cyt (Intra), IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-TRIM25/EFP antibody [EPR7315] (ab167154) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of TRIM25/EFP?
Anti-TRIM25/EFP [EPR7315] (ab167154) specifically detects a band for TRIM25/EFP (UniProt: Q14258) at a molecular weight of 71kDa.
Trusted by the scientific community
Anti-TRIM25/EFP [EPR7315] (ab167154) was first used in a scientific publication in 2013 and has been cited over 30 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-TRIM25/EFP antibody [EPR7315] (ab167154) has been confirmed by Western blot testing in TRIM25 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EPR7315] also available for your convenience: ab167154, Alexa Fluor® 488 -
ab200781
, HRP -
ab200788
, Carrier free -
ab232356
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRIM25 also known as EFP is a protein involved in antiviral response mechanisms. TRIM25 has a molecular mass of approximately 70 kDa and is part of the TRIM (Tripartite Motif) family which contains three distinct zinc-binding domains followed by a coiled-coil region. This protein exhibits strong expression in a variety of tissues including lung liver and intestines. As a member of the E3 ubiquitin ligase enzyme family TRIM25 plays a direct role in protein ubiquitination which tags specific proteins for degradation by the proteasome.
Biological function summary
TRIM25 functions as a significant modulator of the innate immune response against viral infections. In cells it acts within the RIG-I signaling complex directly promoting the RIG-I-MAVS pathway that detects viral RNA. TRIM25 mediates the ubiquitination of RIG-I allowing for its proper activation and subsequent signal transduction facilitating a robust antiviral state. TRIM25's role extends beyond antiviral defenses to include regulatory functions in cell proliferation and differentiation.
Pathways
TRIM25 engages actively in the innate immune signaling and JAK-STAT pathways. It interacts and cooperates with RIG-I in the recognition and response to viral tRNA leading to the production of interferons that establish an antiviral environment. Furthermore TRIM25 also connects with proteins like TRIM21 which further engages the immune response. This interaction enhances the overall ability of cells to fend off viral pathogens efficiently.
Altered TRIM25 function is associated with conditions like systemic lupus erythematosus (SLE) and various cancers. In the context of SLE TRIM25's regulatory role in innate immunity can affect the severity and progression of this autoimmune disorder. Additionally in some cancers abnormal TRIM25 expression can influence cell cycle regulation and metastasis. Through its ubiquitination activities TRIM25 interacts directly with proteins such as p53 affecting tumor suppression pathways and contributing to the complexity of disease development and progression.