Abcam, ab167401, Anti-NELF-B antibody [EPR11200]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal NELF-B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11200,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NELF-B also known as Negative Elongation Factor B functions mechanically as part of the NELF complex that interacts with RNA polymerase II to pause transcription elongation. NELF-B's mass is about 66 kDa. This protein primarily expresses in the nucleus of cells particularly in tissue types where transcription regulation is essential like neurons and muscle cells which require precise gene expression control.
Biological function summary
NELF-B plays a role in regulating gene expression by pausing RNA polymerase II during early elongation. It operates as part of the larger NELF complex which also includes instigates the pausing mechanism. The NELF complex allows for the fine-tuning of transcription and is important for processes involving cell differentiation and response to external stimuli.
Pathways
NELF-B integrates within transcriptional regulation and RNA processing pathways. It directly affects the transcription cycle by contributing to the controlled release of RNA polymerase II from the paused state. NELF-B relates to proteins like DSIF (DRB Sensitivity-Inducing Factor) which collaborates with the NELF complex to modulate transcription dynamics and ensure proper gene expression regulation.
Aberrant function or expression of NELF-B links to cancer and cardiac hypertrophy. In cancer the dysregulation of transcriptional pausing by NELF-B can lead to unchecked cell proliferation. NELF-B also interacts with proteins like MYC where altered expression can amplify oncogenic pathways. In cardiac hypertrophy improper transcription regulation impacts heart muscle cell function emphasizing NELF-B's role in maintaining cellular homeostasis.