Abcam, ab167715, Recombinant HIV-1 gp120 (Du172.17 (mutated E46G, T396A + A497T) protein

Size: 100µg
Recombinant HIV-1 gp120 (Du172.17 (mutated E46G, T396A + A497T) protein is a HIV-1 M:B_HXB2R Full Length protein, in the 36 to 507 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, ELISA.
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by its binding ability in a functional ELISA. Immobilized ab167715 at 5 μg/mL (100 μL/well) can bind Recombinant human CD4 protein (Active) (ab167756) with a linear range of 1-39 ng/mL.,
Accession:P04578,
Animal free:No,
Carrier free:Yes,
Species:HIV-1 M:B_HXB2R,
Reconstitution:Reconstitute at 200 µg/mL in water,
Storage buffer:pH: 7.4Constituents: PBS

Product details:
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
C for 3 months under sterile conditions after reconstitution.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HIV-1 gp120 protein also known as glycoprotein 120 is an essential component of the HIV envelope protein complex. It is a surface protein expressed on the viral envelope and has a molecular mass of approximately 120 kDa. As part of the Env glycoprotein gp120 plays a critical role in virus entry into host cells by mediating attachment to the CD4 receptor on target cells primarily helper T cells.
Biological function summary
The HIV-1 gp120 protein facilitates viral attachment and entry into host cells. It forms a part of the gp160 precursor which cleaves into two subunits: gp120 and gp41 and together they create the HIV envelope spike complex. Gp120 specifically interacts with the CD4 receptor and subsequently co-receptors such as CCR5 or CXCR4 triggering fusion of the viral and cellular membranes through the gp41 protein.
Pathways
The HIV-1 gp120 protein initiates events critical to the human immunodeficiency virus infection process. It plays a role in the viral attachment and entry pathway which is necessary for HIV to penetrate host immune cells. Gp120's interaction with CD4 and co-receptors like CCR5 is essential for the establishment of infection integrating it into pathways involved in immune evasion and pathogenesis.
HIV-1 gp120 is closely associated with the pathogenesis of acquired immunodeficiency syndrome (AIDS). Its role in mediating viral attachment leads to the depletion of CD4-positive T cells a hallmark of AIDS progression. The interaction between gp120 and host proteins like CD4 and CCR5 not only helps in viral entry but also occasionally triggers immune responses or immune exhaustion intensifying disease severity in some individuals.