Abcam, ab170171, Alexa Fluor® 488 Anti-Cleaved PARP1 antibody [4B5BD2]

Size: 100µL
Mouse Monoclonal PARP1 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4B5BD2,
Isotype:IgG1,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab170171 reacts with the N-terminal end formed by the cleavage adjacent to Asp214; it thus recognizes the apoptosis-specific 89 kDa catalytic domain fragment, but it does not recognize the full-length PARP1 or the 24 kDa DNA binding domain fragment.

Product details:
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Precipitation Ammonium Sulphate, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cleaved PARP1 also known as cPARP is a fragment of the PARP1 protein an important DNA repair enzyme. The full PARP1 protein has a molecular weight of approximately 116 kDa but after cleavage during apoptosis the cleaved PARP1 fragments typically have a molecular weight of around 89 kDa and 24 kDa. PARP1 is expressed abundantly in the cell nucleus where it plays important roles in maintaining genomic integrity. The cleavage of PARP1 is a common marker for cell apoptosis pointing towards its breakdown in response to cellular stress.
Biological function summary
The enzymatic function of PARP1 involves the transfer of ADP-ribose units from NAD+ to target proteins a process known as ADP-ribosylation. PARP1 operates as a part of the base excision repair complex essential in DNA repair processes. The cleaved form of PARP1 no longer facilitates DNA repair marking a shift towards apoptosis. When PARP1 is cleaved it indicates caspase activity implying cells are undergoing programmed cell death.
Pathways
Cleaved PARP1 is deeply involved in the apoptosis and DNA damage response pathways. In the apoptosis pathway PARP1 interacts with key proteins like caspase-3 which cleaves PARP during apoptosis. In the DNA damage response PARP1 collaborates with proteins such as XRCC1 facilitating the base excision repair pathway important for fixing single-strand DNA breaks. These pathways highlight the dual role of PARP1 in promoting cell survival through repair and cell death via apoptosis.
Cleaved PARP1 serves as an important marker in cancer and neurodegenerative diseases. In cancer research elevated levels of cleaved PARP1 suggest increased rates of apoptosis in response to anti-cancer therapies linking it to tumor suppression efforts. In neurodegenerative diseases excessive activation and cleavage of PARP1 can result in cell death exacerbating conditions like Alzheimer's disease. Through these contexts cleaved PARP1 connects to other therapeutic targets such as caspase proteins in cancer and to potential PARP inhibitors in neurodegenerative disorders.