Abcam, ab17107, Anti-Laminin alpha 5/LAMA5 antibody [4C7]

Size: 250µL
Mouse Monoclonal Laminin alpha 5/LAMA5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human LAMA5.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4C7,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human LAMA5.O15230

Product details:
This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Laminin alpha 5 (LAMA5) also known as laminin alpha-5 chain is an important component of the basement membrane which provides structural support to tissues. LAMA5 is a subunit of the laminin protein a large heterotrimeric glycoprotein complex involved in numerous cellular processes. The molecular weight of laminin alpha 5 can reach approximately 400 kDa. This protein is expressed widely across various tissues including the kidney lung and skin where it plays a mechanical role by mediating cell adhesion differentiation and migration by forming a scaffold-like structure at the cellular level.
Biological function summary
Laminin alpha 5 participates in the assembly of the laminin-511 complex which is vital for tissue-specific functions and maintaining cellular structures. This complex is important for epithelial and endothelial cell stability ensuring cohesive interactions with integrins and other cellular receptors. Laminin alpha 5 as part of this complex promotes development wound healing and angiogenesis impacting cell survival and regeneration by providing essential biochemical signals through its binding sites.
Pathways
Laminin alpha 5 significantly influences the PI3K/AKT and MAPK pathways which are critical for cell proliferation and survival. Within these pathways LAMA5 interacts with integrin receptors facilitating signal transduction. These pathways further associate laminin alpha 5 with proteins like FAK and Src kinase which mediate downstream signaling events influencing cellular growth and adhesion. These pathways link laminin alpha 5 to diverse cellular functions and regulatory processes.
Laminin alpha 5 has implications in diseases such as renal fibrosis and epidermolysis bullosa. Aberrant expression or mutations of LAMA5 can lead to defective basement membrane integrity affecting tissue stability and function. In renal fibrosis alterations in LAMA5 associate with disrupted kidney architecture while in epidermolysis bullosa laminin alpha 5 mutations lead to skin fragility. These conditions can also involve interactions with proteins like collagen IV and integrins contributing to the disease pathology.