Abcam, ab171744, Anti-PKLR antibody [EPR11093]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PKLR antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11093,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PKLR gene encodes the pyruvate kinase liver and red blood cell enzyme also known as PK-LR. This enzyme catalyzes the last step in the glycolytic pathway converting phosphoenolpyruvate (PEP) into pyruvate with the production of ATP. The alternative names for PKLR include R-type pyruvate kinase and L-type pyruvate kinase. The enzyme has a molecular mass of approximately 57 kDa. It is primarily expressed in the liver and red blood cells with some presence in the pancreatic islets supporting its physiological roles in these tissues.
Biological function summary
Pyruvate kinase is important for energy production by regulating glycolysis. It exists as a homo-tetramer forming a complex to execute its enzymatic activity. This complex ensures the efficient conversion of PEP facilitating the flow of carbon through the glycolytic pathway. In the liver PKLR plays a role in gluconeogenesis and lipogenesis while in red blood cells it contributes directly to ATP production essential for maintaining cellular energy and function.
Pathways
The pyruvate kinase enzyme is a significant participant in glycolysis and gluconeogenesis. These pathways are central to energy metabolism linking glucose breakdown with ATP generation. Pyruvate kinase interacts with enzymes like hexokinase and phosphofructokinase within the glycolytic pathway coordinating with these enzymes to regulate energy metabolism. In gluconeogenesis PKLR integrates into the mechanism ensuring the balance between energy production and storage.
PKLR mutations can lead to pyruvate kinase deficiency a condition that causes hemolytic anemia. This disorder results from impaired ATP production in red blood cells causing them to break down prematurely. In liver tissues dysregulation of PKLR activity associates with cancers like hepatocellular carcinoma. This enzyme connects with proteins such as lactate dehydrogenase in cancer metabolism affecting the Warburg effect which is tangled with cancer cell survival and proliferation.