Abcam, ab171825, Anti-Factor VIII antibody

Size: 50µg
Mouse Polyclonal Factor VIII antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human F8.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human F8.P00451

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Factor VIII also known as anti-hemophilic factor is a glycoprotein with a molecular mass of approximately 250 kDa. This protein circulates in the plasma mainly in the liver where hepatocytes produce it. Factor VIII remains inactive while circulating in the blood and exists within the factor VIII/von Willebrand factor complex. Upon activation it plays a significant role in the blood coagulation process.
Biological function summary
Factor VIII greatly contributes to hemostasis by serving as a cofactor for factor IXa forming the intrinsic tenase complex. This activation results in the conversion of factor X to factor Xa which is important for the common pathway of the coagulation cascade. By participating in the clotting process factor VIII ensures proper wound healing and prevention of blood loss.
Pathways
Factor VIII activity is pivotal within the coagulation cascade particularly the intrinsic and common pathways. Activation of the intrinsic pathway begins through interactions with factor IXa leading to efficient activation of factor X. This process closely relates factor VIII to proteins like factor IX and factor X ensuring proper clot formation and stabilization. Factor VIII products and assays are available to study and characterize its critical role in these pathways.
Factor VIII is closely associated with hemophilia A a genetic bleeding disorder resulting from deficiency or dysfunction of this protein. Patients with hemophilia A exhibit prolonged bleeding times due to impaired coagulation. The disorder links directly to von Willebrand factor as it protects factor VIII from degradation in circulation stabilizing its presence in the blood. Anti-factor VIII antibodies are important tools for diagnosing and studying factor VIII-related disorders.