Abcam, ab171941, Anti-FGF21 antibody [EPR8314(2)]

Size: 20µL / 100µL / 1mL
Anti-FGF21 antibody [EPR8314(2)] (ab171941) is a rabbit monoclonal antibody detecting FGF21 in Western Blot, IHC-P . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 80 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8314(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen used for this product shares 6 continuous identical amino acids with SIKE1. Cross-reactivity with this protein has not been confirmed experimentally.Expression levels of the target protein vary with sample type and some optimisation may be required (PMID: 27285327). For western blot using cell lines, it may be necessary to collect cell culture supernatant for endogenous FGF21 detection as the target protein is readily secreted (PMID: 24041694; PMID: 26691139).ab171941 is not recommended for mouse WB or human IHC-P.

Product details:
What is this antibody validated in?
Anti-FGF21 antibody [EPR8314(2)] (ab171941) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of FGF21?
Anti-FGF21 [EPR8314(2)] (ab171941) specifically detects a band for FGF21 (UniProt: Q9NSA1) at a molecular weight of 22kDa.
Trusted by the scientific community
Anti-FGF21 [EPR8314(2)] (ab171941) was first used in a scientific publication in 2013 and has been cited over 80 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-FGF21 antibody [EPR8314(2)] (ab171941) has been confirmed by Western blot testing in FGF21 Knockout HeLa cell line,
ab265974
Other related products
We have a range of other formats of antibody clone [EPR8314(2)] also available for your convenience: ab171941, Carrier free -
ab219368
, Carrier free -
ab271925
, Alexa Fluor® 488 -
ab322214
, Alexa Fluor® 647 -
ab322215
, Alexa Fluor® 555 -
ab322216
, Alexa Fluor® 594 -
ab322217
, PE -
ab322218
, APC -
ab322219
, Alexa Fluor® 750 -
ab322220
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Allow to warm to room temp and agitate gently before aliquoting, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.
Biological function summary
FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.
Pathways
FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.
FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.