Abcam, ab171973, Anti-ARHGAP27 antibody [EPR12250]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal ARHGAP27 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12250,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARHGAP27 also known as Rho GTPase-activating protein 27 is a regulatory protein with a mass of approximately 88 kDa. It modulates the activity of Rho family GTPases turning them from an active GTP-bound state to an inactive GDP-bound state. This protein mainly expresses in the brain and testis suggesting its specialized roles in these tissues. By controlling the actin cytoskeleton ARHGAP27 influences cell shape motility and division.
Biological function summary
The regulation of cytoskeletal dynamics involves ARHGAP27 in cellular processes. It plays a part in endocytosis a process important for internalizing molecules from the cell surface. ARHGAP27 functions in coordination with other GAPs (GTPase-activating proteins) to achieve its role. It does not operate singularly but can interact within a larger protein complex to exert its function effectively.
Pathways
ARHGAP27 participates within the regulation of Rho GTPase cycle pathway. This pathway is significant for actin cytoskeleton organization. ARHGAP27 interfaces with other proteins like Cdc42 and Rac1 which play pivotal roles in signal transduction related to cytoskeletal arrangements and cellular responses. The protein's modulation of these GTPases impacts intracellular signaling cascades that adjust the organization of actin filaments.
ARHGAP27 associations relate to neurodevelopmental conditions and certain cancers. Mutations or dysregulation of ARHGAP27 may contribute to improper neural connections or tumor development. In some cancer studies researchers identify links between ARHGAP27 and the oncogenic protein RhoA. The interactions and pathways involving ARHGAP27 provide insights into its contributions to pathological states highlighting its potential as a biomarker or therapeutic target.