Abcam, ab172755, Anti-CARD15/NOD2 antibody

Size: 50µg
Mouse Polyclonal CARD15/NOD2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NOD2.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human NOD2.Q9HC29

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The CARD15/NOD2 protein also known as nucleotide-binding oligomerization domain-containing protein 2 is an important protein involved in immune response. It has a molecular mass of about 115 kDa. CARD15/NOD2 expresses mostly in immune cells such as monocytes macrophages and dendritic cells. Additionally it presence can be found in epithelial cells lining the gastrointestinal tract where it plays a vital role.
Biological function summary
CARD15/NOD2 detects microbial components and triggers immune responses. It primarily acts as a pattern recognition receptor identifying bacterial muramyl dipeptide (MDP). Upon activation CARD15/NOD2 initiates a signaling cascade leading to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) which is an important transcription factor in inflammation and immune response. The protein does not operate alone and involves complexes to enable its functions effectively.
Pathways
The CARD15/NOD2 protein engages directly in the NF-kB signaling pathway pivotal for inflammatory and immune responses. It also interacts in the MAPK pathway that participates in cellular responses to stress. CARD15/NOD2 works alongside other proteins such as RIP2 which is a kinase that further transmits signals downstream in these pathways enhancing immune regulation.
Defects or mutations in CARD15/NOD2 relate to inflammatory diseases such as Crohn's disease and Blau syndrome. In Crohn's disease mutations cause an improper immune response to intestinal flora contributing to inflammation. In these conditions CARD15/NOD2 interactions with proteins like RIP2 become altered disrupting normal cellular functions and exacerbating disease symptoms.