Abcam, ab172972, Anti-ME3 antibody [EPR10378]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal ME3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 12 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10378,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ME3 also known as malic enzyme 3 or mitochondrial NADP+-dependent malic enzyme plays an important role in cellular metabolism. It catalyzes the oxidative decarboxylation of malate to pyruvate generating NADPH in the process. The protein has a molecular mass of approximately 65 kDa. ME3 is mainly expressed in tissues with high metabolic demands such as the liver and adipose tissue where it supports various biosynthetic pathways.
Biological function summary
ME3 influences cellular redox balance via its production of NADPH an important reducing agent. It is not part of a larger protein complex but serves an essential function on its own. ME3 contributes to lipid biosynthesis by supplying NADPH for fatty acid elongation and cholesterol synthesis which supports cell growth and proliferation. Its activity impacts overall metabolic homeostasis.
Pathways
ME3 has a significant role in the tricarboxylic acid (TCA) cycle and lipid metabolism. It interfaces with the TCA cycle by providing a critical link between carbohydrate and lipid metabolism through pyruvate production. ME3 interacts with other metabolic enzymes like citrate synthase emphasizing its integrating function in energy production and storage pathways.
ME3's dysregulation associates with metabolic conditions such as obesity and non-alcoholic fatty liver disease (NAFLD). Altered ME3 activity can impact fatty acid metabolism and contribute to lipid accumulation in liver cells. It also interacts with proteins like acetyl-CoA carboxylase involved in fatty acid biosynthesis highlighting its potential role in disease progression. Understanding ME3's function offers insights into the etiology of these metabolic disorders and opportunities for therapeutic intervention.