Abcam, ab173300, Anti-TUFM antibody [EPR12797(B)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal TUFM antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12797(B),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), IHC-P, ICC/IF, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TUFM also known as elongation factor Tu mitochondrial (EF-Tu) is a protein involved in the protein synthesis process within mitochondria. It has a molecular mass of approximately 50 kDa. TUFM plays an essential role in the translation step of protein synthesis by binding aminoacyl-tRNA and positioning it on the ribosome. Researchers have identified the protein as highly expressed in tissues with high energy demand such as the heart brain and skeletal muscle.
Biological function summary
TUFM assists in mitochondrial protein synthesis which is necessary for the proper function and maintenance of the respiratory chain complexes. It forms part of the mitochondrial ribosome complex operating alongside other elongation factors like EF-G. TUFM’s activity is essential for the production of proteins encoded by mitochondrial DNA which are they key components of oxidative phosphorylation machinery.
Pathways
TUFM integrates into the mitochondrial translation pathway which supports the production of proteins critical for the electron transport chain. This pathway is important for ATP production through oxidative phosphorylation. TUFM interacts with other components such as MRPS18 and MRPL3 to mediate the elongation cycle during mitochondrial protein synthesis. These relationships are essential for coordinating mitochondrial function and bioenergetics.
TUFM mutations or disruptions have connections to mitochondrial disorders such as MELAS syndrome (Mitochondrial Encephalomyopathy Lactic Acidosis and Stroke-like episodes) which affects energy production. The protein's dysfunction can also relate to Parkinson's Disease where impaired mitochondrial activity plays a role. TUFM’s interaction with other mitochondrial proteins like NDUFS1 part of complex I of the electron transport chain underlines its potential impact in these disorders.