Abcam, ab174070, Recombinant Human HIV-1 gp120 (CN54) protein

Size: 100µg
Recombinant Human HIV-1 gp120 (CN54) protein is a Fragment protein, in the 34 to 506 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Immobilized Human CD4 at 2 μg/mL (100 μL/well) can bind HIV-1 GP120 (ab174070), His Tag with a linear range of 0.5-63 ng/mL,
Animal free:No,
Carrier free:No,
Reconstitution:Reconstitute with sterile deionized water to a concentration of 400 µg/ml.,
Storage buffer:pH: 7.4Constituents: PBS, 5% Trehalose

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIV-1 gp120 (CN54) also known as glycoprotein 120 is a surface protein expressed on the envelope of the Human Immunodeficiency Virus type 1. The protein has a molecular mass of around 120 kilodaltons. Mechanically gp120 plays a critical role in HIV's ability to infect host cells. It binds to the CD4 receptor on host T-cells and other immune cells. This interaction facilitates the fusion of the viral envelope with the host cell membrane allowing viral entry and subsequent infection.
Biological function summary
Gp120 is essential for viral recognition and binding to the host's immune cells. It is a part of the larger HIV envelope glycoprotein complex along with gp41 forming a trimeric unit that is pivotal for the virus's function. gp120's ability to interact with both the CD4 receptor and co-receptors like CCR5 or CXCR4 defines the tropism of the virus for specific cell types. This targeting mechanism enhances the virus's ability to evade the immune system contributing to the persistence of infection.
Pathways
Gp120 is heavily involved in the entry pathway of the HIV virus into host cells. The primary pathway includes attachment to the CD4 receptor followed by interaction with a coreceptor commonly CCR5 or CXCR4 leading to fusion and viral entry. These interactions are critical in the HIV infection cycle and are targeted by therapeutic interventions. Additionally the involvement of gp41 in the envelop complex is important for the fusion process working closely with gp120.
Gp120 is directly linked to HIV and its pathogenesis in causing Acquired Immunodeficiency Syndrome (AIDS). It facilitates the initial viral entry that leads to the systemic infection associated with the disease. The interaction between gp120 and CD4 receptors plays a significant role in the decline of T-cell populations a hallmark of AIDS. Furthermore gp120's relationship with CCR5 co-receptor also connects it to conditions involving CCR5 such as CCR5-tropic HIV infections guiding therapeutic strategies that aim to block these interactions.