Abcam, ab174406, Recombinant Human Ndfip1 protein (His tag)

Size: 100µg
Recombinant Human Ndfip1 protein (His tag) is a Human Fragment protein, in the 1 to 116 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9BT67,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ndfip1 also known as Nedd4 family interacting protein 1 serves important roles in cellular processes by acting as an adaptor protein that facilitates interaction between Nedd4 family E3 ubiquitin ligases and their substrates. Ndfip1 has a mass of approximately 30 kDa and contains PY motifs which are essential for binding with WW domains of Nedd4 ligases. This protein is ubiquitously expressed but shows higher expression levels in neuronal tissues and immune cells. As Ndfip1 is vital for the regulation of protein degradation it regulates multiple signaling pathways through substrate ubiquitination.
Biological function summary
Ndfip1 regulates intracellular protein levels and maintains cellular homeostasis. By interacting with Nedd4 ligases Ndfip1 assists in the ubiquitination of target proteins marking them for degradation by the proteasome. Ndfip1 does not form a complex itself but rather acts as a facilitator for the formation of ubiquitin ligase complexes. Through this mechanism Ndfip1 affects processes such as endocytosis transcriptional regulation and signal transduction.
Pathways
Ndfip1 plays a significant role in the ubiquitin-proteasome pathway where it aids the degradation of specific proteins to regulate cell signaling. It directly connects with Nedd4 proteins within this pathway affecting substrates that include growth factor receptors and signaling kinases. Another pathway involving Ndfip1 is the immune response pathway where it modulates cytokine receptor turnover impacting immune cell behavior and inflammation.
Ndfip1 is linked to neurodegenerative diseases and immune disorders. Its dysfunction has been studied in the context of Parkinson's disease where misregulation of ubiquitination processes can contribute to neuronal damage. Additionally Ndfip1 associations with immune response pathways suggest its involvement in inflammatory disorders through regulation of cytokine signaling. In these conditions altered interaction with proteins like Nedd4 and cytokine receptors underlies its contribution to disease mechanisms.