Abcam, ab174443, Human IFN gamma ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human IFN gamma (IFNG) ELISA kit is a single-wash, 90-minute SimpleStep ELISA® a used to quantify human interferon-gamma (IFN-γ) with a sensitivity of 470 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450 nm readout; compatible with any standard plate reader - Available in different formats to meet various throughput needs, including 384-well plates for higher throughput - Broad sample compatibility – suitable for serum, plasma (citrate, EDTA), and cell culture supernatants - Cited in over 25 publications
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 470 pg/mL,
Range:0.468 - 30 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human IFN gamma ELISA Kit ab174443 is a rapid single-wash 90-min ELISA kit to measure Human IFN gamma in cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 470 pg/mL.
How the assay works
Human IFN gamma SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This IFN-γ ELISA Kit recognizes both native and recombinant human Interferon-gamma protein in serum, plasma, and cell culture supernatant samples only. Urine, milk, and cell and tissue extract samples have not been tested with this kit.
Human IFN gamma ELISA Kit ab174443 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IFN gamma ELISA Kit ab174443
Human IFN gamma ELISA Kit ab174443 has been used to study inflammation in different physio-pathological contexts such as celiac disease
, pancreatic cancer
and atherosclerosis
References:
Soroush Sardari et al. 2023, PMID: 38040898,
Satoshi Hirano et al. 2023, PMID: 36765558,
Youjiang Qiu, et al. 2023, PMID: 36820569
Save your precious samples
Our 384-well Human IFN gamma ELISA Kit only requires a maxmum of 12.5µL
Related and recommended products
Human IFN gamma ELISA Kit ab174443 is commonly used to measure IFNg ELISA as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human IL-6 ELISA kit
ab178013
- Human TNF alpha ELISA kit
ab181421
- Human IL-1 2 ELISA kit
ab270883
To measure IFN gamma at a lower dynamic range (12.5-1600 pg/mL):
- Human IFN gamma High Sensitivity ELISA kit
ab236895

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary
This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naпve T cells into Th1 cells which is essential for effective cellular immunity.
Pathways
IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.
IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.