Abcam, ab174606, Anti-TLR4/MD2 antibody [MTS510] - Low endotoxin, Azide free

Size: 100µg
Rat Monoclonal MD2 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:MTS510,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Mouse,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TLR4/MD2 also known as Toll-like receptor 4 and myeloid differentiation factor 2 acts as a critical component within the immune system. The TLR4/MD2 complex forms through the interaction between the TLR4 protein and the accessory molecule MD2 enabling recognition of lipopolysaccharides (LPS) from Gram-negative bacteria. TLR4 is a transmembrane protein expressed on various immune cells such as macrophages and dendritic cells. The molecular weight of TLR4 is approximately 96 kDa. Its localization on immune cells allows it to respond promptly to pathogens and initiate necessary immune responses.
Biological function summary
TLR4/MD2 plays a major role in the innate immune response by forming part of a receptor complex. This complex recognizes pathogen-associated molecular patterns and triggers immune signaling that results in the production of pro-inflammatory cytokines. The activation of TLR4/MD2 initiates a cascade of events that enhance the immune system’s ability to fight infections effectively. By signaling through downstream adaptor proteins like MyD88 and TRIF TLR4/MD2 mediates the activation of NF-κB and interferon-regulatory factors leading to gene expression changes and immune activation.
Pathways
The TLR4/MD2 complex aligns with critical signaling pathways involved in inflammation and immune regulation. It holds a significant position in the MyD88-dependent and TRIF-dependent pathways impacting the NF-κB signaling cascade. The interaction with proteins like MyD88 links TLR4/MD2 to the inflammatory response. These pathways reinforce TLR4/MD2's central role in modulating the immune response to protect the host from pathogens while maintaining homeostasis.
TLR4/MD2 exhibits a connection to conditions involving excessive inflammation such as sepsis and rheumatoid arthritis. In sepsis the overactivation of TLR4/MD2 leads to systemic inflammation and potential organ failure. Rheumatoid arthritis manifests chronic inflammation in part due to altered TLR4 signaling. The interaction with other proteins like TRIF is integral to understanding the mechanisms by which TLR4/MD2 contributes to these diseases. Studies target these pathways for therapeutic intervention aiming to modulate TLR4/MD2 activity to alleviate disease symptoms.