Abcam, ab175125, Anti-ZNRF1 antibody

Size: 200µL
Goat Polyclonal ZNRF1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Znrf1 aa 50-150.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Znrf1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.Q91V17,
Specificity:ab175125 is expected to recognize reported isoforms a and b (NP_573469.1 and NP_001162093.1). Reported variants represent identical protein (NP_001162092.1 and NP_573469.1).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab175125 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 99% Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZNRF1 also known as Zinc and Ring Finger 1 is an E3 ubiquitin-protein ligase weighing around 39 kDa. This protein directs the attachment of ubiquitin to target substrates marking them for degradation by the proteasome. ZNRF1 shows expression in the central nervous system and peripheral tissues indicating its significant role in cellular processes across diverse tissue types.
Biological function summary
The cellular function of ZNRF1 involves the regulation of protein stability and turnover affecting a range of physiological processes. ZNRF1 interacts with several key signaling proteins and often functions in coordination with other ubiquitylation enzymes though not classified as part of a specific larger protein complex. Its activity is essential for modulating neuronal survival and regeneration particularly after injury or during neurodegenerative conditions.
Pathways
The activity of ZNRF1 influences the neurotrophin signaling and Wnt pathways. It regulates the degradation of specific neuronal proteins connecting it functionally to proteins such as GAP-43 within the neurotrophin pathway. In the Wnt pathway ZNRF1 impacts protein degradation processes that affect cellular responses to extracellular signals and synaptic function.
ZNRF1 has relevance in neurodegenerative conditions for example Alzheimer's disease where its dysfunction might contribute to neuronal death. It also relates to peripheral neuropathies where the protein's dysregulation impacts nerve regeneration. ZNRF1's role in these diseases connects it with proteins like tau in Alzheimer's and ErbB receptors in neuropathies highlighting its influence on disease progression and potential therapeutic targeting.