Abcam, ab175328, Anti-Rho A + B + C antibody [1A11-4G10]

Size: 100µg
Mouse Monoclonal RHOA antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RHOA.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1A11-4G10,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human RHOA.P61586

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rho proteins including Rho A B and C are small GTPases and part of the larger Rho family sometimes referred as Ras homologous proteins. They have a mass of approximately 21 kDa and play an important role in actin cytoskeleton dynamics. These proteins are primarily expressed in various tissues but are especially prevalent in the brain heart and lungs. When activated Rho proteins bind to GTP facilitating the reorganization of actin fibers critical for processes like cell movement and division.
Biological function summary
Rho proteins influence cell shape migration and adhesion. They are part of the Rho GTPase family which interacts with other molecules to form a complex that manages cellular structure. Rho proteins impact the formation of stress fibers and focal adhesions which are essential for maintaining tissue integrity and facilitating cellular responses. This activity makes Rho proteins vital players in wound healing and embryonic development.
Pathways
Rho proteins are critical parts of the Rho-ROCK (Rho-associated protein kinase) pathway and the Wnt signaling pathway. Both pathways are important for cell signaling that orchestrates changes in the cytoskeleton and cell behavior. In these pathways Rho proteins interact with kinases like ROCK1 and ROCK2 connecting to a larger network of signaling proteins that guide cellular outcomes involved in proliferation and structural maintenance.
Rho proteins have connections to cancer progression and cardiovascular diseases. Their role in cell adhesion and migration links them to metastasis where their dysregulation can promote tumor invasion. Also in cardiovascular conditions altered Rho activity impacts vascular smooth muscle contraction and endothelial function. Research associations identify proteins like E-cadherin in cancer and vascular endothelial cadherin in heart diseases as being influenced by Rho protein signaling.