Abcam, ab175372, Anti-EKLF / KLF1 antibody [1B6A3]

Size: 100µL
Mouse Monoclonal EKLF / KLF1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KLF1 aa 200 to C-terminus.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1B6A3,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human KLF1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q13351

Product details:
This product was changed from ascites to supernatant. Lot no's high than GR252532-8 are from Tissue Culture Supernatant

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EKLF also known as KLF1 is a transcription factor critical for erythropoiesis. It weighs approximately 42 kDa and is predominantly expressed in erythroid cells within the bone marrow. EKLF binds specifically to CACCC elements in the DNA which are sequences commonly found in the promoters of genes essential for red blood cell differentiation. Its ability to regulate gene expression in these cells is instrumental in fetal and adult hemoglobin production. EKLF's distinctive role places it at the heart of erythroid lineage specification and maturation.
Biological function summary
The functions of EKLF extend beyond simple transcriptional regulation. This protein facilitates the switch from fetal to adult hemoglobin by promoting the expression of adult globin chains. EKLF does not work alone; it interacts with co-factors and enhancers to form transcriptional complexes that regulate a diverse array of target genes. These complexes are necessary for the execution of gene networks that include the alpha- and beta-globin gene clusters. Such regulation by EKLF is pivotal for maintaining erythroid cells' identity and function.
Pathways
EKLF integrates itself within the erythropoiesis and heme biosynthesis pathways. EKLF activates genes that participate in the erythropoietin signaling and heme production both of which are essential for effective erythrocyte maturation and function. EKLF also coordinates with other important transcription factors such as GATA1 and FOG-1. These interactions elucidate how EKLF aligns with broader gene expression networks emphasizing its essential role in processes leading to efficient red blood cell production.
EKLF has a significant link to β-thalassemia and other hemoglobinopathies. Mutations or dysregulation within EKLF can disrupt normal hemoglobin synthesis leading to these disorders. Notably EKLF's regulation of hemoglobin gene expression connects it to diseases in tissues outside of the erythroid system. Furthermore EKLF interacts with proteins such as BCL11A in the context of β-thalassemia. This relationship highlights potential therapeutic strategies that target these pathways to treat hemoglobin disorders effectively.