Abcam, ab175424, Anti-EDR1 / PHC1 antibody [1F3F3]

Size: 100µg
Mouse Monoclonal EDR1 / PHC1 antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PHC1 aa 750 to C-terminus.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1F3F3,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PHC1 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P78364

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EDR1 also known as PHC1 is a protein that functions mechanically by participating in chromatin modification. Its molecular mass is approximately 105 kDa. The protein is expressed in various tissues with significant presence noted in proliferative cells. PHC1 is commonly referred to as a member of the Polycomb group proteins which are essential in regulating gene expression by modifying chromatin structure influencing the accessibility of DNA for transcription. It plays a critical role in the maintenance of repression states of homeotic genes during development.
Biological function summary
EDR1/PHC1 is involved in maintaining cellular identity and proliferation by forming part of the Polycomb repressive complex 1 (PRC1). This complex facilitates the ubiquitination of histone H2A leading to transcriptional repression. PHC1 influences the silencing of specific target genes that are important in developmental pathways. It also plays a role in the regulation of stem cell pluripotency and differentiation contributing to the epigenetic memory of cells that persist through cell divisions.
Pathways
PHC1 significantly influences gene silencing through its involvement in the Polycomb repressive complex 1 pathway interacting with other Polycomb-related proteins like RING1 and BMI1. The protein is key in the regulation of the Wnt signaling pathway impacting the balance between cell differentiation and proliferation. PHC1 affects this pathway by modulating the expression of genes that are important for cell fate determination. Its interaction with BMI1 and other Polycomb group proteins ensures the proper progression of these pathways.
Defects in PHC1 contribute to leukaemias and neural developmental disorders. Mutations within the PHC1 gene have been associated with disrupted gene silencing and aberrant expression profiles contributing to the etiology of these conditions. In leukaemias its interaction with BMI1 is important as the dysregulation of this complex can lead to uncontrolled cell proliferation. In neural developmental disorders PHC1 affects the expression of key regulatory genes further highlighting its importance in normal cellular function.