Abcam, ab176106, Anti-HuB+HuC+HuD antibody [16A11C1D4]

Size: 100µg
Mouse Monoclonal HuC/HuD protein antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ELAVL2 aa 200-250 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:16A11C1D4,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ELAVL2 aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q12926

Properties and Storage Information:
Form-Liquid, Purification notes-The purity of ab176106 is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation., Storage buffer-Preservative: 0.02% Sodium azideConstituents: 99% HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HuB HuC and HuD proteins often referred to collectively as Hu antigens are neuronal RNA-binding proteins. They belong to the ELAVL (Embryonic Lethal Abnormal Vision in Drosophila) protein family. These proteins have masses typically ranging from around 35-42 kDa. They are expressed chiefly in the nervous system where they play critical roles in the regulation of mRNA. HuB (also known as ELAVL2) HuC (ELAVL3) and HuD (ELAVL4) support neuronal differentiation and maintenance.
Biological function summary
The Hu antigens stabilize and modulate the translation of target mRNAs impacting processes such as neuronal growth and synaptic plasticity. These proteins do not exist in isolation; they are integrated into RNP (ribonucleoprotein) complexes in neurons. HuC and HuD in particular serve as important neuronal markers often used in research to identify neuronal lineage and differentiation. Hu proteins influence gene expression by binding to AU-rich elements located in the 3' untranslated region of mRNAs.
Pathways
HuB HuC and HuD play a significant role in the post-transcriptional regulation pathway influencing the stability and translation of mRNAs involved in neuronal function. This is important for learning and memory processes where proteins like FMRP are also involved. HuD for instance is linked with pathways that regulate mRNA turnover and translation initiation affecting how neural cells respond to various stimuli.
Alterations in HuD expression levels have links to neurodegenerative diseases such as Alzheimer's disease. Abnormal interactions between HuD and tau protein which is implicated in Alzheimer's suggest pathways where Hu proteins contribute to disease progression. Schizophrenia also demonstrates associations with improper Hu protein functioning where disruptions in mRNA binding and stabilization may affect neural communication and brain development.