Abcam, ab176317, Anti-LIMPII antibody [EPR12080]

Size: 20µL / 100µL / 1mL
Anti-LIMPII antibody [EPR12080] (ab176317) is a rabbit monoclonal antibody detecting LIMPII in Western Blot, IHC-P . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12080,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-LIMPII antibody [EPR12080] (ab176317) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of LIMPII?
Anti-LIMPII [EPR12080] (ab176317) specifically detects a band for LIMPII (UniProt: Q14108) at a molecular weight of 54kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-LIMPII antibody [EPR12080] (ab176317) has been confirmed by Western blot testing in SCARB2 Knockout MCF7 cell line,
ab274952
Other related products
We have a range of other formats of antibody clone [EPR12080] also available for your convenience: ab176317, Carrier free -
ab240186
, PE -
ab305994
, APC -
ab305995
, HRP -
ab305996
, Alkaline Phosphatase -
ab308926
, Alexa Fluor® 488 -
ab309856
, Alexa Fluor® 647 -
ab310224
, Alexa Fluor® 594 -
ab310671
, Alexa Fluor® 555 -
ab312202
, Alexa Fluor® 568 -
ab312690
, Alexa Fluor® 750 -
ab321184
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LIMPII also known as LIMP-2 is a lysosomal membrane protein involved in the transport and degradation processes within cells. It has an approximate molecular mass of 54 kDa. LIMPII is mainly expressed in the lysosomal membranes of a variety of tissues notably in the kidney liver and spleen. Its structural characteristics enable it to function effectively within these cellular organelles delivering enzymes and other molecules essential for cellular maintenance and function.
Biological function summary
The protein plays a critical role in lipid and protein degradation and helps maintain cellular homeostasis. As part of the lysosomal membrane LIMPII facilitates the uptake and processing of large biomolecules into the lysosome. It functions as a receptor for the uptake of Β-glucocerebrosidase forming a complex to assist in its proper delivery from the endoplasmic reticulum to the lysosomes. This receptor-ligand relationship ensures efficient enzymatic activity necessary for lipid metabolism.
Pathways
One finds that LIMPII participates in the sphingolipid metabolism pathway and is vital for glucosylceramide catabolism. It interacts with glucocerebrosidase in this pathway which plays a significant role in the metabolism of glycosphingolipids. Another pathway where LIMPII has importance is the autophagy pathway where it contributes to the fusion of autophagosomes with lysosomes. Its activity supports the turnover of cellular components maintaining cellular metabolic balance.
LIMPII's dysfunction has links to Gaucher's disease and atherosclerosis. Both conditions relate to improper lipid metabolism. In Gaucher's disease the interaction between LIMPII and glucocerebrosidase becomes disrupted leading to lysosomal storage issues. In contrast altered expressions of LIMPII correlate with atherosclerosis possibly due to its role in lipid processing. These associations suggest that LIMPII could be a significant factor in therapeutic approaches for these diseases.