Abcam, ab176464, Anti-HIF3 alpha/IPAS antibody

Size: 100µg
Rabbit Polyclonal HIF3 alpha/IPAS antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HIF3A aa 450-550.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human HIF3A aa 450-550. The exact immunogen used to generate this antibody is proprietary information.Q9Y2N7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIF3 alpha also known as IPAS stands as a unique member of the hypoxia-inducible factor (HIF) family involved in oxygen regulation. HIF3 alpha is a transcription factor made of approximately 66 kDa protein mass which plays a role in controlling gene expression in response to low oxygen conditions. It gets expressed in various tissues including the brain lungs and kidneys. Unlike HIF1 alpha and HIF2 alpha HIF3 alpha can act as negative regulator of HIF-mediated gene activation by forming complexes with other HIFs impeding their transcriptional ability.
Biological function summary
HIF3 alpha engages in cellular response to hypoxic environments by binding to hypoxia-responsive elements in DNA. It forms heterodimers with ARNT an aryl hydrocarbon receptor nuclear translocator allowing interaction with DNA sequences needed for hypoxia response. HIF3 alpha differs by sometimes inhibiting rather than activating target genes providing a balance to the functions of other HIFs. It affects vascular development and energy metabolism performing roles outside traditional hypoxia response.
Pathways
HIF3 alpha integrates into key biological networks such as the HIF signaling pathway and oxygen homeostasis. Within these pathways it interacts with other HIF family members including HIF1 alpha and HIF2 alpha regulating downstream hypoxia-adaptive responses. This interaction allows a fine-tuned balance in the expression of genes linked to angiogenesis and erythropoiesis exemplifying its antagonistic function in comparison to other HIFs that usually activate these pathways.
HIF3 alpha assumes significant roles in the context of conditions like non-small cell lung carcinoma (NSCLC) and sleep apnea. Studies suggest alterations in HIF3 alpha expression contribute to tumor progression in NSCLC and the regulation of respiratory drive under low oxygen conditions in sleep apnea. Through these disease processes potential connections with proteins like VEGF (Vascular Endothelial Growth Factor) underline HIF3 alpha's modulatory impact in angiogenic activity and hypoxic responses affecting disease progression and outcomes.