Abcam, ab176645, JNK 1/2 (pT183/Y185) ELISA Kit

Size: 1 x 96Tests
JNK 1/2 (pT183/Y185) ELISA Kit is a single-wash 90-min Simplestep used to quantify JNK 1/2 (pT183/Y185) with a sensitivity of 0.5 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Cited in over 5 citations
Key facts
Detection method:Colorimetric,
Sample types:Tissue Homogenate, Cell Lysate,
Reacts with:Mouse, Human,
Assay type:Semi-quantitative,
Sensitivity:= 0.5 ng/mL,
Range:1 - 100 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Abcam's JNK1/2 (pT183/Y185)
in vitro
SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of JNK1/2 (pT183/Y185) protein in Human and mouse cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
As of October 2019, this kit was reformulated with new antibodies to maintain continued long term supply.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
JNK1 and JNK2 are part of the c-Jun N-terminal kinase group also called Stress-activated protein kinases (SAPKs). These proteins are serine/threonine kinases with a known molecular weight around 46-54 kDa depending on their phosphorylation state. JNK1 and JNK2 phosphorylate specific target proteins which include transcription factors and other kinases to regulate various cellular processes. They are expressed in most tissues with higher levels in brain heart and skeletal muscle. JNK isoforms have become essential in research involving stress responses where specific tools such as JNK antibodies and Western blot assays targeting p-JNK molecular weight are critical for study.
Biological function summary
These enzymes play vital roles in regulating cellular responses to stress stimuli. JNK1 and JNK2 form part of larger signaling complexes including the JNK molecular weight complex that is activated in stressful conditions like UV radiation or cytokines. Once activated they phosphorylate transcription factors such as c-Jun leading to gene expression changes that facilitate adaptative and survival responses. They modulate cellular processes like apoptosis inflammation and cell differentiation clearly contributing to homeostasis and development.
Pathways
These kinases interact significantly within the MAPK (Mitogen-activated protein kinase) and apoptosis pathways. JNK proteins mediate signals from upstream kinases such as MKK4/7 and respond to inflammatory cytokines resulting in transcriptional alterations. Their downstream impact is tightly linked to apoptosis through interactions with Bcl-2 family members affecting cellular fate. ERK and p38 MAPK proteins closely interact with JNK pathways co-regulating cellular stress responses and survival pathways highlighting their importance in maintaining cellular balance.
The altered regulation of JNK1 and JNK2 links them to diverse conditions such as cancer and neurodegenerative diseases. In cancer abnormal JNK activity affects cell proliferation and apoptosis with JNK pathways often hyperactivated in tumors. In neurodegeneration these kinases contribute to cell death as seen in disorders like Alzheimer's disease where they engage with proteins like tau leading to neuronal apoptosis. The connection of JNK proteins to other stress-activated kinases further positions them as critical therapeutic targets in disease modulation.