Abcam, ab177203, Anti-MKRN3 antibody

Size: 100µg
Rabbit Polyclonal MKRN3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MKRN3 aa 250-450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MKRN3 aa 250-450. The exact immunogen used to generate this antibody is proprietary information.Q13064

Properties and Storage Information:
Form-Lyophilized, Reconstitution-reconstitute with water at 200µL, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MKRN3 also known as Makorin Ring Finger Protein 3 is an important protein characterized by its E3 ubiquitin ligase activity. This protein has a mass of approximately 43 kDa. MKRN3 is expressed in the hypothalamus and is highly relevant during developmental stages before puberty. It interacts with RNA-binding proteins and other molecular components within cells indicating its mechanistic action in post-translational modification and regulation of cellular processes.
Biological function summary
MKRN3 regulates the puberty onset. It is a part of an intricate network controlling gene expression related to this process. MKRN3 functions as a negative regulator and suppresses the release of puberty-activating signals. This places it within a tightly coordinated biological context where it collaborates with various factors to maintain the prepubertal state. Researchers identify it as an essential player in the hypothalamic-pituitary-gonadal axis.
Pathways
Several cascading signals are affected by MKRN3. It plays a role in the reproductive endocrine axis specifically influencing the GnRH (Gonadotropin-Releasing Hormone) signaling pathway. MKRN3's involvement in these pathways links it with other associated proteins like KISS1 and its receptor which play significant roles in triggering puberty. The regulatory capacity of MKRN3 means it acts as an upstream modulator within these signaling mechanisms.
MKRN3 mutations lead to central precocious puberty. This condition involves early activation of the hypothalamic-pituitary-gonadal axis causing early puberty onset. Additionally altered MKRN3 expression may relate to hypogonadotropic hypogonadism a disorder characterized by delayed or absent puberty. In this context MKRN3 connects with genes and proteins such as GNRHR and TAC3 known for their roles in reproductive development disorders making it necessary for scientists to explore MKRN3's potential as a therapeutic target.