Abcam, ab17771, Anti-procollagen type IIA antibody [2]

Size: 100µg / 100µL
Mouse Monoclonal Collagen II antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:Epitope is located at the amino-terminal part of procollagen type IIA.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, 2.9% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Procollagen type IIA also known as COL2A1 is a precursor of type II collagen important in forming fibrillar collagen networks. This protein has a molecular mass of approximately 146 kDa. Its expression occurs largely in cartilaginous tissues particularly during the early stages of chondrogenesis. Procollagen type IIA is also found in non-skeletal tissues such as the eye indicating its diverse roles during organismal development.
Biological function summary
Procollagen type IIA contributes to the structural integrity of cartilaginous matrices supporting tissue framework and providing tensile strength. Procollagen type IIA is a precursor in the collagen synthesis pathway where it undergoes a series of enzymatic modifications before converting into mature type II collagen fibrils. This protein forms part of a larger collagen synthesis complex and interacts with enzymes like procollagen N-proteinase playing an essential role in matrix formation.
Pathways
Procollagen type IIA participates in the extracellular matrix (ECM) organization pathway. This involvement is key in skeletal development and tissue repair. The protein interacts closely with other structural components like procollagen type IIB to support chondrogenic activities. Also it is part of the transforming growth factor-beta (TGF-beta) signaling pathway which regulates chondrocyte function and differentiation.
Procollagen type IIA mutations are linked to skeletal disorders such as stickler syndrome and achondrogenesis type II. These mutations disrupt normal collagen fiber assembly affecting cartilage function and leading to distinct skeletal abnormalities. In these disorders it is interconnected with proteins like matrix metalloproteinases (MMPs) which influence tissue remodeling and degradation exacerbating the disease conditions.