Abcam, ab177941, Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]

Size: 20µL / 100µL / 1mL
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941) is a rabbit monoclonal antibody detecting OGT / O-Linked N-Acetylglucosamine Transferase in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12713,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Product details:
What is this antibody validated in?
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of OGT / O-Linked N-Acetylglucosamine Transferase?
Anti-OGT / O-Linked N-Acetylglucosamine Transferase [EPR12713] (ab177941) specifically detects a band for OGT / O-Linked N-Acetylglucosamine Transferase (UniProt: O15294) at a molecular weight of 116kDa.
Trusted by the scientific community
Anti-OGT / O-Linked N-Acetylglucosamine Transferase [EPR12713] (ab177941) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR12713] also available for your convenience: ab177941, Alexa Fluor® 647 -
ab198532
, Alexa Fluor® 555 -
ab215809
, Carrier free -
ab236014
, PE -
ab305958
, APC -
ab305959
, HRP -
ab305960
, Alkaline Phosphatase -
ab308846
, Alexa Fluor® 488 -
ab309777
, Alexa Fluor® 594 -
ab310575
, Alexa Fluor® 568 -
ab312586
, Alexa Fluor® 750 -
ab321209
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OGT or O-Linked N-Acetylglucosamine Transferase also known as OGT protein mechanically catalyzes the addition of N-acetylglucosamine (O-GlcNAc) onto serine or threonine residues of nuclear and cytoplasmic proteins. OGT has a molecular weight of approximately 110 kDa. It is expressed in various tissues with higher levels in the pancreas brain and spleen. This enzyme plays an important role in cellular processes by modifying proteins with O-GlcNAc.
Biological function summary
Through its transferase activity OGT regulates a wide array of cellular functions including signal transduction transcription and metabolism. It is a component of a complex involving several other proteins that facilitate its regulatory actions. OGT manages protein function and stability by influencing interactions between proteins and other cellular components.
Pathways
OGT is critical in the insulin signaling pathway and the regulation of glucose metabolism. It interacts with multiple proteins such as IRS-1 and Akt to modulate their activity and stability. The modification by OGT impacts protein phosphorylation states affecting the downstream processing of cellular signals.
OGT has a significant link to diabetes and neurodegenerative diseases like Alzheimer's. Abnormal OGT activity relates to insulin resistance contributing to diabetes pathogenesis. In Alzheimer's disease altered O-GlcNAcylation affects proteins like tau which play a central role in disease progression. Understanding OGT's role in these conditions could aid in developing targeted therapeutic strategies.