Abcam, ab178423, Anti-SDHB antibody [EPR13042(B)]

Size: 100µL / 1mL
Anti-SDHB antibody [EPR13042(B)] (ab178423) is a rabbit monoclonal antibody detecting SDHB in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13042(B),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IP, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-SDHB antibody [EPR13042(B)] (ab178423) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of SDHB?
Anti-SDHB [EPR13042(B)] (ab178423) specifically detects a band for SDHB (UniProt: P21912) at a molecular weight of 32kDa.
Trusted by the scientific community
Anti-SDHB [EPR13042(B)] (ab178423) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-SDHB antibody [EPR13042(B)] (ab178423) has been confirmed by Western blot testing in SDHB Knockout HEK-293 cells.
Other related products
We have a range of other formats of antibody clone [EPR13042(B)] also available for your convenience: ab178423, Carrier free -
ab250046
, PE -
ab305931
, APC -
ab305932
, HRP -
ab305933
, Alkaline Phosphatase -
ab308925
, Alexa Fluor® 488 -
ab309855
, Alexa Fluor® 647 -
ab310223
, Alexa Fluor® 594 -
ab310670
, Alexa Fluor® 555 -
ab312201
, Alexa Fluor® 568 -
ab312689
, Alexa Fluor® 750 -
ab321213
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein SDHB also known as Succinate Dehydrogenase Complex Iron Sulfur Subunit B plays a mechanical role as a component of the succinate dehydrogenase (SDH) enzyme. This subunit has a molecular mass of approximately 30 kDa. SDHB is part of the mitochondrial inner membrane where it is expressed in high levels across various tissues including heart kidney and muscle. It contributes to the electron transport chain by facilitating the oxidation of succinate to fumarate an important step in energy production through the tricarboxylic acid (TCA) cycle.
Biological function summary
SDHB participates in the succinate dehydrogenase complex acting within both the TCA cycle and the electron transport chain. By transferring electrons to coenzyme Q it helps generate an electrochemical gradient important for ATP synthesis. This complex also called Complex II includes other subunits such as SDHA SDHC and SDHD. Their interactions ensure proper function of metabolic processes within mitochondria bridging the gap between foundational energy metabolism and complex cellular processes.
Pathways
SDHB plays an important role in cellular respiration and metabolic cycles. It resides in the TCA cycle and the mitochondrial electron transport chain connecting its function to energy generation pathways. Specifically SDHB relates closely to SDHA and coenzyme Q all working together to facilitate electron transfer and effective mitochondrial energy output. The transfer of electrons through this pathway highlights its essential contribution to maintaining cellular energy homeostasis.
SDHB mutations are associated with conditions such as paraganglioma and pheochromocytoma. These genetic alterations disrupt normal complex II function leading to the accumulation of succinate and succinate-related oncogenesis. In addition to causing tumor development defective SDHB is linked with familial paraganglioma syndromes. Its interaction with proteins such as SDHA and SDHD critical for the succinate dehydrogenase functionality highlights its significant role in maintaining metabolic balance and when defective contributing to disease pathogenesis.