Abcam, ab178440, Anti-Ref2P antibody

Size: 100µg
Anti-Ref2P antibody (ab178440) is a rabbit polyclonal antibody detecting Ref2P in Western Blot . Suitable for Drosophila melanogaster . - Over 40 publications
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Drosophila melanogaster,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Ref2P antibody (ab178440) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Drosophila melanogaster samples.
What is the molecular weight of Ref2P?
Anti-Ref2P (ab178440) specifically detects a band for Ref2P (UniProt: P14199) at a molecular weight of 65kDa.
Trusted by the scientific community
Anti-Ref2P (ab178440) was first used in a scientific publication in 2013 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Ref2P (ab178440) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ref(2)P also known as Ref(2)P protein or Recycling Endosome Factor 2 Protein plays a significant role in autophagy in Drosophila. This protein is approximately 65 kDa in mass and is expressed widely in various tissues including neuronal and muscle tissues. Mechanically Ref(2)P functions as a scaffold protein facilitating the formation and degradation of protein aggregates. It also interacts with other key components of the autophagy machinery to promote the selective degradation of ubiquitinated proteins.
Biological function summary
This protein assists in maintaining cellular homeostasis by targeting damaged or unnecessary proteins for degradation. Ref(2)P serves as the fly homolog of the mammalian p62/SQSTM1 and is recognized as an adaptor protein within the autophagic machinery. It binds ubiquitinated proteins and interacts with LC3 a core component of the autophagosome aiding in the clearance of protein aggregates. This process is essential for protecting cells especially those under stress conditions.
Pathways
Ref(2)P integrates into the autophagy-lysosome pathway which is important for cellular recycling processes. It collaborates with key proteins like Atg1 and Atg8. Additionally the protein impacts the TOR signaling pathway. In a nutrient-rich environment TOR can inhibit autophagy while Ref(2)P acts in response to cellular stress promoting autophagy and improving metabolic homeostasis.
Abnormalities in Ref(2)P expression relate strongly to neurodegenerative diseases including conditions similar to Alzheimer's. Ref(2)P malfunctions can lead to the accumulation of protein aggregates which are harmful to neurons. This protein also connects indirectly to disorders involving the ubiquitin-proteasome system since it functions in tagging proteins for autophagic degradation. Therapeutic strategies targeting Ref(2)P or its pathways could potentially alleviate symptoms linked to these diseases.