Abcam, ab178658, Human Luteinizing hormone ELISA Kit (LH)

Size: 1 x 96Tests
Human Luteinizing hormone ELISA Kit (LH) is a sandwich ELISA designed to quantify Human Luteinizing hormone (LH) with a sensitivity of 0.22 mU/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 5 - 200 mU/mL
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.22 mU/mL,
Range:5 - 200 mU/mL,
Assay Platform:Microplate

Product details:
Human Luteinizing hormone ELISA Kit (LH) ab178658 is a sandwich ELISA to measure Human Luteinizing hormone (LH) in serum, citrate plasma, heparin plasma with a sensitivity of 0.22 0.
How the assay works
A 96-well plate has been precoated with Streptavidin. Samples, standards and the LH-HRP and Bitoin conjugate are added to the wells. Biotinylated monoclonal and enzyme labeled antibodies are added and the reactants mixed: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of LH. Reaction between the various LH antibodies and native LH occurs in the microwells without competition or steric hindrance forming a soluble sandwich complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of LH in the sample and the intensity is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Luteinizing hormone ELISA Kit (LH) ab178658 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Add prepared Conjugation Solution to appropriate wells. Incubate at room temperature.
3. After washing, add TMB Substrate Solution to each well. Incubate at room temperature.
4. Add Stop Solution. Read at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C