Abcam, ab178846, Anti-Iba1 antibody [EPR16588] - Microglia marker

Size: 100µL / 1mL
Anti-Iba1 antibody [EPR16588] (ab178846) is a rabbit monoclonal antibody detecting Iba1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF . Suitable for Human, Mouse, Rat . - Clone EPR16588 is the most cited clone to Iba1 - Biophysical QC for unrivalled batch-batch consistency - Over 430 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16588,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-Fr, ICC/IF, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:For ab178846 Abcam recommends blocking in 3% milk for cleanest results in WB. Blocking with BSA gives slightly higher background.Iba1 is a relatively minor protein of brain and is much more abundant in spleen (PMID: 8912632, PMID: 29232670). We suggest loading higher amount of brain lysate or using lower dilution of antibody for detecting signal in brain related lysates

Product details:
Anti-Iba1 antibody [EPR16588] (ab178846) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Anti-Iba1 antibody [EPR16588] (ab178846) was first used in a scientific publication in 2016 and has been cited over 439 times in peer reviewed journals. It's performance in IHC, Western Blot and Immunofluorescence in mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Iba1 antibody [EPR16588] (ab178846) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Iba1 antibody [EPR16588] (ab178846) has 44 independent reviews from customers.
Anti-Iba1 antibody [EPR16588] (ab178846) specifically detects Iba1 (UniProt ID: O70200; Molecular weight: 17kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR16588 -
ab220815
Antibody clone EPR16588 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, APC, Alexa Fluor
ab225260
ab225261
ab320071
ab321218
NEW: Explore our alternative host species for clone [EPR16588] : goat, chicken, rat. Iba1 (AIF1) is a marker for microglia, the brain's primary immune cells and is frequently used in Alzheimer's disease (AD) research. In AD brains, altered Iba1 expression indicates microglial activation and neuroinflammation. Researchers use Iba1 to assess microglial response and motility in AD models, making it essential for understanding the role of inflammation and immune response in Alzheimer's pathology.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Iba1 also known as allograft inflammatory factor 1 (AIF1) is a protein with a molecular weight of approximately 17 kDa. It is widely expressed in microglia which are the resident immune cells of the central nervous system (CNS). Iba1 plays a mechanical role in actin-binding and modulating cytoskeleton dynamics which affects cell shape and motility. It is also found in other myeloid-derived cells including macrophages and monocytes where it influences cellular processes linked to inflammation and immune responses.
Biological function summary
The involvement of Iba1 in actin polymerization impacts microglia's ability to perform phagocytosis and cytokine production. Iba1 does not appear to be part of any large protein complex but interacts directly with actin filaments to facilitate cytoskeletal reorganization. This function enables the cells to move and respond efficiently to tissue damage or pathogens in the CNS. It plays an important role in supporting cellular changes associated with immune surveillance and neuroinflammatory responses.
Pathways
Microglial activation relies heavily on Iba1 for executing acts linked with neuroinflammation. Iba1 engages in the NF-κB signaling pathway significant in managing inflammatory responses. It interacts with proteins such as MyD88 and IRAK4 which aid in transmitting signals from toll-like receptors. These pathways enable changes in gene expression critical for microglial response to various stimuli. Furthermore Iba1 also contributes to the PI3K/Akt pathway where it influences cell survival and proliferation interacting with proteins like Akt1 and PTEN.
Iba1 has implications in neurodegenerative diseases notably Alzheimer's disease and multiple sclerosis. In Alzheimer's disease increased expression of Iba1 is observed in activated microglia around amyloid plaques suggesting a role in plaque clearance and neuroinflammation. The protein relates to amyloid precursor protein (APP) as microglia respond to abnormal accumulations. In multiple sclerosis Iba1 expression marks activated microglia involved in demyelination processes where it associates with proteins like myelin basic protein (MBP) highlighting its role in the disease's pathology.