Abcam, ab178850, Anti-Choline Acetyltransferase antibody [EPR16590]

Size: 20µL / 100µL / 1mL
Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) is a rabbit monoclonal antibody detecting Choline Acetyltransferase in Western Blot, IHC-P . Suitable for Guinea pig, Mouse, Rabbit, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 50 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16590,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Rabbit, Guinea pig,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Product Specifications
Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, WB in guinea pig, mouse, rabbit, rat samples.
Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) specifically detects Choline Acetyltransferase (UniProt ID: P13222; Molecular weight: 72kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) has been cited over 54 times in peer reviewed journals and is trusted by the scientific community.
Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) has 5 independent reviews from customers.
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Target Information
Choline Acetyltransferase (ChAT) is an enzyme crucial for the synthesis of acetylcholine, a neurotransmitter involved in many functions, including muscle activation and cognitive processes. It serves as a marker for cholinergic neurons in research. Impaired ChAT activity is implicated in various conditions, including Alzheimer's disease (AD), where decreased acetylcholine levels lead to cognitive deficits, and myasthenia gravis, an autoimmune disorder that disrupts neuromuscular communication.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.
Biological function summary
The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.
Pathways
Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.
Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.