Abcam, ab178945, Anti-iNOS antibody [EPR16635]

Size: 20µL / 100µL / 1mL
Anti-iNOS antibody [EPR16635] (ab178945) is a rabbit monoclonal antibody detecting iNOS in Western Blot, IP, ICC/IF, ELISA . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 270 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16635,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human, Rat,
Applications:ICC/IF, WB, I-ELISA, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody shows low affinity on human samples.Based on our preliminary data, this antibody is not suitable for THP1 (Human monocytic leukemia monocyte) cell lines in WB.

Product details:
Anti-iNOS antibody [EPR16635] (ab178945) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in I-ELISA, ICC/IF, IP and WB.
Anti-iNOS antibody [EPR16635] (ab178945) was first used in a scientific publication in 2014 and has been cited over 275 times in peer reviewed journals. It's performance in Western Blot in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-iNOS antibody [EPR16635] (ab178945) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-iNOS antibody [EPR16635] (ab178945) specifically detects iNOS (UniProt ID: P35228; Molecular weight: 132kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR16635 -
ab213987
Antibody clone EPR16635 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
647, Alexa Fluor
555, Alexa Fluor
ab209027
ab209594
ab209595
Inducible nitric oxide synthase (iNOS) plays a key role in disease by producing nitric oxide (NO), which can contribute to inflammation and tissue damage in conditions like arthritis, sepsis and cancer. EPR16635 is one of the top cited recombinant antibodies on the market with over 494 citations.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.
Biological function summary
INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.
Pathways
INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.
INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.