Abcam, ab179515, Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]

Size: 20µL / 100µL / 1mL
Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) is a rabbit monoclonal antibody detecting pro Caspase-1 + p10 + p12 in Western Blot, IP . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 310 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16883,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody has low affinity in Ms/Rt brain, H1299 and A549. 55kDa and 80kDa nonspecific bands may be observed in some samples.

Product details:
Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IP and WB.
Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) was first used in a scientific publication in 2017 and has been cited over 310 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) specifically detects pro Caspase-1 + p10 + p12 (UniProt ID: P29466; Molecular weight: 20kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR16883 -
ab238972
Pro-caspase-1, along with its subunits p10 and p12, plays a critical role in the activation of the inflammasome, which is involved in inflammatory responses and is linked to various diseases, including autoimmune disorders and infections
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
'Pro Caspase-1' is an inactive precursor of Caspase-1 also known as ICE (interleukin-1β-converting enzyme). It plays an important role in the process of inflammation and apoptosis. The pro-enzyme undergoes proteolytic processing to produce active Caspase-1 which consists of two subunits p10 and p20. The molecular weight of pro Caspase-1 is approximately 45 kDa. It is expressed in various tissues especially in immune cells like macrophages and monocytes. Expression levels can vary according to the inflammatory status of the tissue.
Biological function summary
Pro Caspase-1 is involved in the innate immune response by regulating the maturation of pro-inflammatory cytokines such as IL-1β and IL-18. This protein is a part of an essential multimeric complex known as the inflammasome. The inflammasome facilitates the activation of pro Caspase-1 leading to its conversion to active Caspase-1 initiating the cleavage and release of cytokines that signal the presence of infections and stress in cells.
Pathways
Pro Caspase-1 is integrated into the inflammation and pyroptosis pathways. It interacts with various proteins like ASC (apoptosis-associated speck-like protein containing a CARD) and NLRP3 to form the inflammasome complex which is important for cytokine processing and cell death mechanisms. These pathways demonstrate its significant role in limiting pathogen invasion and resolving inflammation through regulated cellular responses.
Pro Caspase-1 is connected to several inflammatory conditions including rheumatoid arthritis and inflammatory bowel disease. The activity of Caspase-1 regulated by its pro-form contributes to the pathogenesis of these disorders due to excessive inflammation. Additionally abnormal Caspase-1 activation implicates conditions like familial Mediterranean fever which often involve proteins like Pyrin in the inflammatory cascade. Understanding pro Caspase-1 dynamics helps in developing therapeutic strategies to modulate inflammation in these diseases.