Abcam, ab17987, Anti-Werner's syndrome helicase WRN antibody

Size: 100µg
Rabbit Polyclonal Werner's syndrome helicase WRN antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.1% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Werner's syndrome helicase also known as WRN plays a mechanical role in DNA replication and repair. WRN is a 160 kDa protein containing both helicase and exonuclease activities making it unique among RecQ helicases. It unwinds DNA duplexes and resolves complex DNA structures that can arise during replication and repair processes. WRN is expressed in the nucleus of various cell types including fibroblasts where it maintains genome stability. This helicase is critical in processing DNA intermediates to prevent genomic instability.
Biological function summary
WRN functions as a part of larger protein complexes that participate in DNA damage response and repair. It interacts with several proteins involved in the homologous recombination and base excision repair pathways. WRN's unique enzymatic activities enable it to stabilize and process DNA ends during replication fork collapse and double-strand break repair. By operating in concert with proteins such as RAD51 and replication protein A (RPA) WRN ensures accurate replication and repair preventing mutation accumulation.
Pathways
WRN plays a central role in the maintenance of genomic integrity through its involvement in DNA repair pathways and the aging process. It is integral to the process of homologous recombination where it works closely with the MRE11-RAD50-NBS1 (MRN) complex. Additionally WRN has a function in the DNA damage checkpoint pathway collaborating with proteins like ATM and ATR which help to sense damaged DNA and initiate repair processes. These pathways are important for maintaining DNA integrity and preventing cellular senescence.
WRN is closely linked to Werner syndrome and cancer. Mutations in the WRN gene lead to Werner syndrome a rare disorder characterized by premature aging and cancer predisposition. WRN's interaction with tumor suppressor proteins such as p53 connects it to pathways that can contribute to tumorigenesis when dysregulated. Understanding WRN's function and its role in these diseases provides insight into mechanisms of aging and cancer development offering potential therapeutic targets.