Abcam, ab180140, Anti-APPL antibody [EPR13569]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal APPL antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 10 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13569,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APPL also known as Adaptor Protein Phosphotyrosine Interaction PH Domain and Leucine Zipper Containing 1 (APPL1) is an adaptor protein that plays an important role in cellular signal transduction. APPL1 has a molecular mass of approximately 80 kDa. It is widely expressed in various tissues including the brain liver and skeletal muscle. APPL1 binds to many other proteins via its pleckstrin homology (PH) domain and a leucine zipper domain playing a role in diverse signaling pathways.
Biological function summary
APPL1 influences cellular processes such as endocytosis and cell proliferation. APPL1 interacts with rab5 forming a component of endosomal signaling complexes. This interaction drives the early endocytic process influencing cell surface receptor trafficking. APPL1 also modulates insulin signaling by binding to proteins like the insulin receptor substrate affecting glucose metabolism and cellular energy balance.
Pathways
APPL1 acts in the AKT signaling pathway and the adiponectin signaling pathway. In the AKT pathway APPL1 operates with proteins like AKT itself and GSK3β contributing to cell survival and growth. It assists the adiponectin pathway by facilitating interactions between adiponectin receptors and downstream effectors impacting lipid metabolism and insulin sensitivity. APPL1's involvement in these pathways highlights its multifaceted regulatory roles in cellular metabolism and response to stimuli.
APPL1 exhibits associations with type 2 diabetes and certain cancers. APPL1 influences insulin sensitivity a critical factor in type 2 diabetes pathogenesis. It interacts with proteins like adiponectin and the insulin receptor substrate playing a part in glucose homeostasis. In cancer APPL1's role in AKT signaling can influence tumor growth and survival linking it to dysregulation in cancers such as breast cancer. The ability of APPL1 to modulate key signaling pathways highlights its importance in understanding these diseases.