Abcam, ab18056, Anti-HEF1/NEDD-9 antibody [2G9]

Size: 100µL
Mouse Monoclonal HEF1/NEDD-9 antibody. Suitable for ICC, IP, Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Human NEDD9 aa 50-400.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2G9,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human, Rat, Mouse,
Applications:IHC-P, IP, ICC/IF, ICC, IHC-Fr, WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NEDD9 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.Q14511,
Specificity:Not tested on Sin1. This antibody mostly detects HEF1 / NEDD-9 localized at the focal adhesion sites.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.2Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HEF1 also known as NEDD9 is an intracellular signaling protein and a member of the Crk-associated substrate family. It has a molecular mass of approximately 93 kDa. The expression of this protein occurs mainly in tissues involved in cell movement and proliferation like lymphoid organs and neural tissues. It is part of the CAS (Crk-associated substrate) proteins hence associated with a wide range of cellular mechanics.
Biological function summary
HEF1 functions as a scaffolding protein involved in various cellular processes including cell adhesion migration and proliferation. It interacts with several other proteins and forms part of complexes such as focal adhesion complexes. It regulates the dynamics of these complexes by modulating signaling pathways linked to cellular adhesion and migration. In neuronal cells it influences axonal and dendritic branching.
Pathways
HEF1 plays a significant role in both the integrin signaling pathway and cell cycle regulation. Through these pathways HEF1 interacts with various key proteins including focal adhesion kinase (FAK) and Src kinase. It positions itself strategically within these pathways and facilitates cross-talk between signaling molecules enabling the coordination of structural and replicative functions in cells.
HEF1 has been implicated in the progression of cancers such as breast cancer and melanoma. Its overexpression often correlates with increased metastatic potential. In breast cancer HEF1 interacts with proteins like Aurora A and BRCA1 linking it to pathways involved in tumor proliferation and invasion. In melanoma alterations in HEF1 levels can affect melanoma cell migration making it a potential target for therapeutic intervention.