Abcam, ab180578, Anti-MGAT1 antibody [EPR14247]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal MGAT1 antibody. Suitable for WB and reacts with Human samples. Cited in 14 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14247,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MGAT1 also known as mannosyl (α-13-)-glycoprotein β-12-N-acetylglucosaminyltransferase is an enzyme with a molecular mass of approximately 51 kDa. It is a critical component of the N-glycosylation process in eukaryotic cells. This protein resides primarily in the Golgi apparatus where it acts as a glycosyltransferase enzyme. MGAT1 catalyzes the transfer of N-acetylglucosamine to the mannose residues on the glycan chain which is an important step in forming complex N-glycans. This modification impacts several cellular functions.
Biological function summary
MGAT1 plays an essential role in protein folding stability and function by modifying N-glycans attached to nascent proteins. The enzyme acts as part of a complex that includes other enzymes like MGAT2 MGAT3 and MGAT5 in the glycan biosynthesis pathway. MGAT1's activity significantly influences cell-cell communication signaling and immune responses. Its function enhances the diversity and biological roles of glycoproteins within cells impacting various physiological processes.
Pathways
MGAT1 is a significant player in the protein glycosylation and folding pathways. It interacts with proteins like MGAT2 and galectin-3 indicating its involvement in broader metabolic processes. N-glycan biosynthesis pathway is its primary pathway where it contributes by enabling the elongation and branching of N-glycan structures. This glycosylation process affects cellular processes such as signal transduction and protein trafficking through downstream effects on glycoproteins.
MGAT1 has implications in congenital disorders of glycosylation and cancer. Abnormal MGAT1 activity leads to improper glycosylation causing congenital disorders that affect multiple systems including nervous and digestive systems. In cancer MGAT1-linked glycan alterations on surface proteins contribute to tumor progression and metastasis. Disrupted interactions with proteins like MGAT5 can increase cell surface glycoprotein complexity promoting cancer cell survival and immune evasion.