Abcam, ab180588, Anti-RBPJK antibody [EPR13479]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal RBPJK antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 18 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13479,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RBPJK also known as CSL or CBF1 is a transcriptional regulator that plays an important role in the Notch signaling pathway. It has a molecular mass of approximately 60 kDa. RBPJK expression occurs in multiple tissues including the thymus brain and heart. This protein operates mechanically by binding to DNA and mediating the transcriptional activity of Notch target genes.
Biological function summary
The protein functions as a central component of the Notch signaling complex. It interacts with other proteins to regulate the expression of genes that guide cell fate decisions. RBPJK forms complexes with Notch intracellular domain and Mastermind-like (MAML) co-activators and it can switch between repressor or activator of transcription depending on its binding partners. This regulation ensures proper development and tissue homeostasis.
Pathways
RBPJK influences processes that are critical in cell differentiation and proliferation. It resides within the Notch signaling pathway where it is essential for transmitting Notch receptor signals into the cell nucleus. RBPJK associates with proteins such as Notch1 and MAML1 facilitating a cascade of events important for determining cellular outcomes during developmental processes and maintaining stem cell populations.
Dysregulation of RBPJK has connections to cancer and Alzheimer’s disease. In cancer aberrant Notch signaling often involving RBPJK leads to unchecked cell proliferation and survival making it a target for drug development. Alzheimer's disease studies reveal that RBPJK may influence the processing of amyloid precursor protein with possible links to abnormal protein aggregation. Understanding interactions with proteins like NICD and APP is important for unraveling its role in these conditions.