Abcam, ab180880, Hela-DFO treated (0.5mM, 24h) Nuclear Lysate

Size: 200µg
Hela-DFO treated (0.5mM, 24h) Nuclear Lysate. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Disease:Adenocarcinoma

Product details:
We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.
The cells were treated for 24 hours with a 0.5mM solution of Deferoxamine (DFO). DFO is used as a hypoxia-mimetic agent to stabilize Hypoxia Inducible Factor 1 (HIF-1). DFO stabilizes HIF-1 through the inhibition of Prolyl Hydroxylases (PHDs) which target HIF-1 through degradation. The mechanism of DFO inhibition is likely through the chelation of Fe2+ bound to the active site of PHD which is required for enzymatic activity.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle