Abcam, ab18104, Anti-Ada protein antibody [ADA-1]

Size: 50µg
Mouse Monoclonal ADA antibody. Suitable for IP, WB, RIA and reacts with Escherichia coli samples. Cited in 3 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:ADA-1,
Isotype:Unknown,
Carrier free:No,
Reacts with:Escherichia coli,
Applications:IP, WB, RIASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Ada protein also known as Ada1 plays a significant role in the bacterial adaptive response to alkylation damage. It acts as a DNA methyltransferase specifically responsible for repairing methylated bases in DNA. Ada protein has an approximate mass of 39 kilodaltons. It is expressed within many bacterial species with E. coli being a common example where its function is well-studied. Ada modulates its activity through a two-step process involving the transfer of methyl groups from damaged DNA to its own cysteine and lysine residues.
Biological function summary
Ada protein serves to directly repair methylated DNA lesions which prevents mutation and maintains genetic stability. It often functions as part of a regulatory complex that initiates the expression of other DNA repair enzymes. The Ada protein acts as both a sensor and a regulator by increasing the transcription of genes involved in the adaptive response making it a central player in the cellular defense against alkylating agents.
Pathways
Ada protein's function links closely with the DNA damage repair pathways. It primarily associates with the base excision repair (BER) pathway to correct lesions caused by endogenous and exogenous agents. Its interaction with other components such as the AlkB protein highlights its role in an interconnected network aiming to maintain the integrity of the genetic material.
Ada protein's impairment correlates with increased mutation rates and potential onset of diseases like cancer due to inadequate DNA repair mechanisms. Although primarily studied in bacterial contexts its analogy in human systems particularly related proteins such as MGMT (O6-methylguanine-DNA methyltransferase) highlights its importance in understanding tumor resistance to alkylating chemotherapy agents. Understanding Ada and similar proteins provide insights into mechanisms that safeguard cellular genomes against mutagenic threats.