Abcam, ab181094, Anti-GCAT antibody [EPR13450] - N-terminal

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal GCAT antibody. N-terminal. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13450,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GCAT also known as glycine C-acetyltransferase functions as a mitochondrial enzyme involved in glycine metabolism. It has a molecular mass of approximately 44 kDa. Researchers often find GCAT expressed in the liver where it plays a significant role in metabolic processes related to amino acid synthesis and catabolism. Being predominantly cytosolic GCAT contributes to the detoxification pathways by participating in the conversion of glycine to other metabolites.
Biological function summary
In glycine cleavage system GCAT operates as part of a complex essential for the regulation of amino acid levels. The enzyme accepts substrates from reactions facilitated by other components such as T-protein and P-protein and is vital in maintaining the balance of glycine and other amino acids within the cell. Dysfunction in any part of this system can result in elevated levels of toxic metabolic intermediates leading to cellular stress and potential tissue damage.
Pathways
GCAT is an integral element in the mitochondrial glycine cleavage system linking it to broader metabolic pathways such as the Krebs cycle and oxidative phosphorylation. This position between pathways underlines its connection with proteins such as glycine dehydrogenase and aminomethyltransferase. This interconnectedness suggests that imbalances involving GCAT can disrupt metabolic homeostasis influencing energy production at the cellular level.
Defects or dysregulation in GCAT activity link to various metabolic conditions including nonketotic hyperglycinemia and liver diseases. Patients with nonketotic hyperglycinemia show accumulation of glycine leading to neurological issues conditions typically associated with deficiencies in glycine metabolism enzymes like P-protein. In the context of liver diseases alterations in GCAT's function affect the liver's ability to process nitrogen highlighting its importance in liver homeostasis and function.