Abcam, ab181153, Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal IREB2/IRP2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 19 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13854,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The antibody immunogen share 80% homology with the IREB1 protein, therefore it is likely that the antibody cross-react with IREB1. We received some customers feedback where they observed this cross-reactivity, however no experiments were performed in our lab in order to confirm this.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IREB2 also known as IRP2 and ACO1 also known as Aconitase 1 serve vital functions in iron metabolism. Mechanically IREB2/IRP2 acts as an iron-responsive element-binding protein and is involved in the post-transcriptional regulation of key genes including those encoding transferrin receptor and ferritin. ACO1 functions as a bifunctional protein that switches between being an enzyme and a regulatory protein based on iron levels known to be around 98 kDa in mass. Both of these proteins express in various tissues highlighting their importance across different biological systems.
Biological function summary
The IREB2/IRP2 and ACO1 maintain cellular iron homeostasis by responding to iron levels and regulating the iron-responsive element system. They do not form part of a larger protein complex but operate independently to modulate iron metabolism. When iron is ample ACO1 behaves as an enzyme catalyzing the isomerization of citrate to isocitrate in the citric acid cycle. Conversely under iron-depleted conditions ACO1 joins IREB2/IRP2 in an RNA-binding form to control iron uptake and storage.
Pathways
These proteins play significant roles in the iron regulatory network and the citric acid cycle. IREB2/IRP2 associates with pathways involving iron uptake and release influencing genes related to ferritin and transferrin receptor. ACO1's dual function ties it to both the citric acid cycle and iron regulatory pathways showing its involvement in cellular energy production and iron balance. The interaction with other important proteins such as transferrin and ferritin is important for their regulatory functions within these pathways.
IREB2 and ACO1 connections are apparent in conditions like neurodegenerative diseases and anemia. Abnormal regulation or mutations in these proteins show associations with disorders such as Parkinson’s disease and sideroblastic anemia. Interactions between IREB2/IRP2 and proteins like PARK7/DJ-1 and oxidative stress proteins underline their significance in the pathogenesis of neurodegenerative diseases and issues of iron mismanagement.